CGSC Strain#: 7451
Strain Designation: CAG18638      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, glgP721::Tn10, rph-1
Strain Comments:

• glgP721::Tn10-- : originated in BW6166 as insertion ca. 16 min. from PO18 of J4; shown by PCR to be within glgP gene, at nucleotide 3,564,175 (76.827 min.)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• glgP721::Tn10 was formerly called zhh-721::Tn10
• glgP721::Tn10 was formerly called zhh-21::Tn10
• = tetracycline resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of