CGSC Strain#: 7426
Strain Designation: CAG12182      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, cysI3152::Tn10kan, rph-1
Strain Comments:

• cysI3152::Tn10kan-- derived from cysI95::Tn10, shown by PCR to be inserted after nucleotide 2,886,785 (minute 62.226) in cysI rather than cysC (Nichols et al.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• cysI3152::Tn10kan was formerly called cysC3152::Tn10kan
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of