CGSC Strain#: 7403
Strain Designation: CAG18577      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, atoS3142::Tn10kan, gyrA261(NalR), rph-1
Strain Comments:

• atoS3142::Tn10kan-- : Cotransduction values for atoS298::Tn10 are 77% with ompC and 30% with gyrA.
• atoS3142::Tn10kan-- derived from atoS298::Tn10, formerly called zej-298::Tn10, shown by PCR to be within atoS at nucleotide 2,318,294(49.97 min.).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• atoS3142::Tn10kan was formerly called zej-3142::Tn10kan
• = kanamycin resistant
• NalR = Nalidix.acidResist.

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.