CGSC Strain#: 7402
Strain Designation: CAG12177      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, atoS298::Tn10, gyrA261(NalR), rph-1
Strain Comments:

• The gyrA allele from the parent CS1230 was added to the genotype following feedback reports and subsequent testing that indicated that the strain is NalR.
• atoS298::Tn10-- : insertion shown by PCR (Nichols et al. 1998) to be within the atoS gene, at nucleotide 2,318,294 (49.972 min.). Originated in Schnaitman strain CS1230.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• atoS298::Tn10 was formerly called zei-298::Tn10
• atoS298::Tn10 was formerly called zeg-298::Tn10
• atoS298::Tn10 was formerly called zeh-298::Tn10
• = tetracycline resistant
• NalR = Nalidix.acidResist.

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.