CGSC Strain#: 7389
Strain Designation: CAG18578      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, zea-3124::Tn10kan, rph-1
Strain Comments:

• zea-3124::Tn10kan-- : derived from zea-225::Tn10, which cotransduces 58% with gap and 42% with fadD, and was placed by PCR (Nichols et al. 1998) in orf b1785(yeaI)at nucleotide 1,869,535 (40.298 min.)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of