CGSC Strain#: 7410
Strain Designation: CAG18468      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, nupC510::Tn10, rph-1
Strain Comments:

• nupC510::Tn10-- originated in strain S\O1024; found by PCR to be inserted at nucleotide 2,511,317 (Nichols et al. 1998, J.Bacteriol. 180:6408).
• nupC510::Tn10-- : 50-80% cotransduction with ptsH1, ptsI2, ptsI27.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = tetracycline resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of