CGSC Strain#: 7450
Strain Designation: CAG18450      Source of Strain: C.A. Gross
Other Designations: CAG22119
Sex: F- Chromosomal Markers: λ^-, zhg-50::Tn10, rph-1
Strain Comments:

• CAG18450 is the same as CAG22119 in Table 3, Berlyn et al.
• zhg-50::Tn10-- is in the intergenic region between ugpB and livF (Nichols et al.) and cotransduces 80% with rpoH.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• zhg-50::Tn10 was formerly called zhf-5::Tn10
• = tetracycline resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Berlyn, M.K.B., K.B. Low, K.E. Rudd 1996. Linkage map of Escherichia coli K-12, Ed.9, with Appendix, Recalibrated Map Positions of Transposon Insertion Sites, M.Singer and K.B.Low. Escherichia coli and Salmonella, Neidhardt et al., ASM ASM:1715-1902
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.