CGSC Strain#: 7248
Strain Designation: PN104      Source of Strain: Marians and P.Nurse
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, lacZ8(Am), phoA4(Am), λ^-, ompF627(T2R), fadL701(T2R), relA1, pitA10, dinD1::Mud(Ap,lac), spoT1, priA1::KanR, rrnB-2, mcrB1, creC510
Strain Comments:

• lacZ8(Am)-- From Miller's CSH manual: carries amber mutation at position 17 of lacZ (Kleina et al. 1990)
• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• dinD1::Mud(Ap,lac)-- promoterless lacZ fused with dinD1 promoter in a defective Mu phage, construct also AmpR, integrated into the chromosome
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• lacZ8(Am) was formerly called lac_ull8am
• Am = amber(UAG) mutation
• Const = constitutive
• T2R = T2 Resistant

References:

• Nurse, P., K.H. Zavitz, K.J. Marians 1991. Inactivation of the Escherichia coli PriA DNA replication protein induces the SOS response. J.Bacteriol. 173:6686-6693