CGSC Strain#: 7247
Strain Designation: PN103      Source of Strain: K.J. Marians
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, phoA4(Am), λ^-, ompF627(T2R), serU132(AS), fadL701(T2R), relA1, pitA10, priA1::KanR, mcrB1, creC510
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• serU132(AS) was formerly called Su1⁺
• serU132(AS) was formerly called supD32
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Const = constitutive
• T2R = T2 Resistant

References:

• Nurse, P., K.H. Zavitz, K.J. Marians 1991. Inactivation of the Escherichia coli PriA DNA replication protein induces the SOS response. J.Bacteriol. 173:6686-6693