CGSC Strain#: 5439
Strain Designation: H12R3a      Source of Strain:
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, phoA37, ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, phoT-35, rrnB-2, mcrB9999, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• phoT-35-- is a phoS or phoT mutation.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

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