CGSC Strain#: 7244
Strain Designation: S\O5060      Source of Strain: P. Nygaard
Sex: F- Chromosomal Markers: lacZ2286(Am), λ^-, purR97, relA1, rpsL150(strR), spoT1
Strain Comments:

• lacZ2286(Am)-- is a UAG mutation in the first segment of the lacZ gene (Zipser et al., 1970).
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Kilstrup, M., L.M. Meng, J. Neuhard, P. Nygaard 1989. Genetic evidence for a repressor of synthesis of cytosine deaminase and purine biosynthesis enzymes in Escherichia coli. J.Bacteriol. 171:2124-2127
• Meng, L.M., M. Kilstrup, P. Nygaard 1990. Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli. Eur.J.Biochem. 187:373-379