CGSC Strain#: 13884
Strain Designation: pLC22-8
Source of Strain: Sex: F+
Episome/Plasmid: pLC22-8A
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1,
argU ,
kdsB ,
ilvG ,
ilvE Plasmid Comment: Mixed clone. Hybridizes with Kohara clones 530, 531, 532, and 629 (consecutive clones). Carries argU ca. 12.1, kdsB ca. 20.9, and ilvGE at 85.1 minutes.. See also pLC22-8B, pLC22-8C, and pLC22-8D.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA56-- Amino acid substitution Arg 60 Cys
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Hubscher, U., A. Kornberg 1979. The delta subunit of Escherichia coli DNA polymerase III holoenzyme is the dnaX gene product. Proc.Natl.Acad.Sci.USA 76:6284-6288
- Henson, J.M., H. Chu, C.I. Irwin, J.R. Walker 1979. Isolation and characterization of dnaX and dnaY temperature sensitive mutants of Escherichia coli. Genetics 92:1041-1059
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151