CGSC Strain#: 7137
Strain Designation: SE53      Source of Strain: K.T. Shanmugam
Sex: F- Chromosomal Markers: leuB6(Am), galT27, λ^-, bioA2, nar-3, srl-202, recA56, hydA-101, rpsL179(strR), thiE1, suc-10
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• nar-3-- is either narG or narH
• recA56-- Amino acid substitution Arg 60 Cys
• hydA-101-- : mutation in the hydA( hydN-hypF) operon
• nar-3 was formerly called chlC3
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Lee, J.H., P. Patel, P. Sankar, K.T. Shanmugam 1985. Isolation and characterization of mutant strains of Escherichia coli altered in H2 metabolism. J.Bacteriol. 162:344-352
• Sankar, P., J.H. Lee, K.T. Shanmugam 1985. Cloning of hydrogenase genes and the fine structure of an operon essential for H2 metabolism in Escherichia coli. J.Bacteriol. 162:353-360