CGSC Strain#: 7112
Strain Designation: RP6666      Source of Strain: S. Parkinson
Sex: F- Chromosomal Markers: araC14, fhuA31, Δ(argF-lac)169, tsx-78, ?motA1214, hisG4(Oc), rfbC1, rpsL136(strR), xylA5, mtl-1, metF159(Am), thiE1
Strain Comments:

• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• ?motA1214-- Deletion of bp 366 to 846 of the motA gene, which is replaced with 104 bp of phage Mu DNA.
• ?motA1214-- is the result of a phage mu excision.
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Slocum, M.K., J.S. Parkinson 1983. Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: organization of the tar region. J.Bacteriol. 155:565-577
• Garza, A.G., P.A. Bronstein, P.A. Valdez, L.W. Harris-Haller, M.D. Manson 1996. Extragenic suppression of motA missense mutations of Escherichia coli. J.Bacteriol. 178:6116-6122