CGSC Strain#: 7169
Strain Designation: PRC79      Source of Strain: D.P. Clark
Sex: F+ Chromosomal Markers: λ^-, hns-206, adhEp-81, oppC506::Tn10, rpoS396(Am), rph-1
Strain Comments:

• adhEp-81-- is an operator or promoter mutation. It results in 60-fold overproduction of ADH on succinate plus acetate medium and allows growth on EtOH as sole C source.
• oppC506::Tn10-- cotransduces 83% with tyrT and 43% with trpA. It was shown to be within the oppC locus by Nichols et al. (1998).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• adhEp-81 was formerly called adhC81
• hns-206 was formerly called bglY206
• oppC506::Tn10 was formerly called zci-506::Tn10
• = tetracycline resistant
• Am = amber(UAG) mutation

References:

• Cunningham, P.R., D.P. Clark 1986. The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol.Gen.Genet. 205:487-493