CGSC Strain#: 7121
Strain Designation: NI512      Source of Strain: H. Nakayama
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, thiL49, glnX44(AS), gal-6, λ^-, thiK48, trp-31, cpxB11, hisG1, rfbC1, thiD45, galP63, argG6, rpsL104, malT1(λ^R), xyl-7, mtl-21, metB1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thiL49-- - Note: thiL thiD double mutant does not respond to thiamin supplementation. It requires 0.1uM TPP for growth in Luria broth.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thiK48-- - Note: a thiK thiD double is unable to grow in the presence of thiamin. Grows in Luria + 0.1 uM thiamin monophosphate.
• cpxB11-- was formerly called cpxB1.
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References: