CGSC Strain#: 6122
Strain Designation: MI250      Source of Strain: M. Iaccarino
Sex: F- Chromosomal Markers: fhuA23, brnR3, brnQ2, tsx-67, purE42, glnX44(AS), λ^-, trpE38, lysA23, brnT8, rpsL109(strR), xylA5, mtl-1, ilvA601, thiE1
Strain Comments:

• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• brnT8 was formerly called brn-8
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• strR = streptomycin resist.

References:

• Guardiola, J., M. DeFelice, T. Klopotowski, M. Iaccarino 1974. Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12. J.Bacteriol. 117:393-405