CGSC Strain#: 7163
Strain Designation: CCMB1005      Source of Strain: Bhargava
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, ompF627(T2R), fadL701(T2R), zfi-905::Tn10, relA1, pitA10, spoT1, pst-9, mcrB1, creC510, lyt-1(ts)
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• pst-9-- pstA or B
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• pst-9 was formerly called phoT9
• = tetracycline resistant
• Const = constitutive
• T2R = T2 Resistant
• ts = Temperature Sensitive

References:

• Chitnis, SN, KS Prasad, PM Bhargava 1990. Isolation and characterization of autolysis-defective mutants of Escherichia coli that are resistant to the lytic activity of seminalplasmin. J. Gen. Microbiol. 136(3):463-9.