CGSC Strain#: 6653
Strain Designation: CBK741      Source of Strain: C.M. Berg
Sex: F- Chromosomal Markers: Δ(pro-lac), thyA0, avt23::Tn5, ilvE12
Strain Comments:

• thyA0-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.

References:

• Whalen, W.A., M.-D. Wang, C.M. Berg 1985. Beta-chloro-L-alanine inhibition of the Escherichia coli alanine-valine transaminase. J.Bacteriol. 164:1350-1351