CGSC Strain#: 4559
Strain Designation: AT1024      Source of Strain: A.L. Taylor
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, glnX44(AS), Rac-0, rfbC1, mgl-51, lysA22, rpsL20(strR), xylA5, mtl-1, thiE1, Lys+
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• lysA22-- is Taylor's lysA10 which he briefly called lysB10 in 1971.
• Lys+-- in presence of lysA22 causes overproduction of Pdx. prototrophic for Lys and Pdx
• Δ(gpt-proA)62 was formerly called proA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.

References:

• Bukhari, A.I., A.L. Taylor 1971. Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine. J.Bacteriol. 105:988-998
• Dempsey, W.B., L.J. Arcement 1971. Identification of the forms of vitamin B6 present in the culture media of "vitamin B6 control" mutants. J.Bacteriol. 107:580-582