CGSC Strain#: 7040
Strain Designation: CA8404      Source of Strain: J.R. Beckwith
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, rpsL136(strR), crp-1004, spoT1, ΔcyaA854, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ΔcyaA854-- is a 200 bp frameshift deletion that generates a new translation start (Glaser et al., below)
• crp-1004 was formerly called cap*
• crp-1004 was formerly called crp-k4
• thiE1 was formerly called thi-1
• strR = streptomycin resist.

References:

• Brickman, E., L. Soll, J.R. Beckwith 1973. Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. J.Bacteriol. 116:582-587
• Sabourin, D, J Beckwith 1975. Deletion of the Escherichia coli crp gene. J. Bacteriol. 122(1):338-40.