CGSC Strain#: 6851
Strain Designation: Hfr 3000 pyr      Source of Strain: F. Jacob
Other Designations: YA231
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: e14-, relA1, spoT1, thiE1, pyrB231
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• pyrB231-- in Hfr 3000 YA231 #6851. 11/85 streaked min glu, B1, + ura --this allele is leaky.
• thiE1 was formerly called thi-1

References:

• Beckwith, J.R., A.B. Pardee, R. Austrian, F. Jacob 1962. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J.Mol.Biol. 5:618-634