CGSC Strain#: 5776
Strain Designation: DEV3      Source of Strain: D. Elseviers
Other Designations: KL16 lac5 strA21
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: lacZ105(Am), e14-, relA1, rpsL222(strR), thiE1
Strain Comments:

• lacZ105(Am)-- is a UAG mutation.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Petrullo, LA, PJ Gallagher, D Elseviers 1983. The role of 2-methylthio-N6-isopentenyladenosine in readthrough and suppression of nonsense codons in Escherichia coli. Mol. Gen. Genet. 190(2):289-94.