CGSC Strain#: 5687
Strain Designation: JRG590      Source of Strain: J.R. Guest
Other Designations: HΔ10
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: secA207(aziR), Δ(nadC-aceF)72, relA1, spoT1, thiE1
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• secA207(aziR) was formerly called azi-7
• thiE1 was formerly called thi-1
• aziR = azide resistance

References:

• Langley, D., J.R. Guest 1977. Biochemical studies of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants. J.Gen.Microbiol. 99:263-276
• Guest, JR 1977. Menaquinone biosynthesis: mutants of Escherichia coli K-12 requiring 2-succinylbenzoate. J. Bacteriol. 130(3):1038-46.