CGSC Strain#: 6923
Strain Designation: GI76-16      Source of Strain: D.W. Grogan
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, lacZ4, glnX44(AS), cfa-4, ksgB1, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• cfa-4-- = IN( 'cfa-zdg299::Tn10')202, which inactivates cfa, results in Tet-sensitivity, and inactivates ksgB1, giving kasugamycin sensitivity.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Grogan, D.W., J.E. Cronan 1984. Genetic characterization of Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci. Mol.Gen.Genet. 196:367-372
• Grogan, D.W., J.E. Cronan 1986. Characterization of Escherichia coli mutants cmpletely defective in synthesis of cyclopropane fatty acids. J.Bacteriol. 166:872-877