CGSC Strain#: 6843
Strain Designation: EJ1321      
Source of Strain: E. Juni
Sex: F- 
 Chromosomal Markers: galK2(Oc)
, 
λ-, 
IN(rrnD-rrnE)1, 
rpsL200(strR)
, 
pck-11, 
maeB3, 
maeA1, 
maeR2Strain Comments: - galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.                                                                                                          
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- maeA1-- affects NAD-linked malic enzyme (EC1.1.1.38) and is unmapped.                                                                                                                                                                                                 
- maeR2-- is a regulatory mutation for malic enzyme                                                                                                                                                                                                                     
- maeB3-- affects NADP-linked malic enzyme (EC1.1.1.40)                                                                                                                                                                                                                 
- galK2(Oc) was formerly called gal2
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References:- Hansen, EJ, E Juni 1975. Isolation of mutants of Escherichia coli lacking NAD- and NADP-linked malic. Biochem. Biophys. Res. Commun. 65(2):559-66.