CGSC Strain#: 6450
Strain Designation: χ2224      Source of Strain: R. Curtiss
Other Designations: chi2224
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), secA208, fhuA2::IS2, lacY1, glnX44(AS), galK2(Oc), minB-2, tdk-2, udk-30, upp-30, rpsL109(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• secA208-- : azi is now secA
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• minB-2-- mini-cell producer
• fhuA2::IS2 was formerly called fhuA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• secA208 was formerly called azi-8
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Maturin, LJ, R Curtiss 1981. Role of ribonucleic acid synthesis in conjugational transfer of chromosomal and plasmid deoxyribonucleic acids. J. Bacteriol. 146(2):552-63.