CGSC Strain#: 6204
Strain Designation: KL325      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: secA209(aziR), pro-48, lacZ608(Am), lacI22, e14-, trpA9605(Am), his-85(Am), gyrA19(NalR), serV43(AS), rpsL171(strR), metE70, trpR55
Strain Comments:

• lyohil opened 9/03
• pro-48-- is probably in proB^-.
• lacZ608(Am)-- is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• e14--- : Loss of the e14 element makes the strain McrA^-.
• trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
• his-85(Am)-- is an amber mutation. It is suppressed by supD32.
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• his-85(Am) was formerly called his-29
• lacI22 was formerly called lac_i3^-
• lacI22 was formerly called lacI3
• secA209(aziR) was formerly called azi-9
• serV43(AS) was formerly called supD43
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• aziR = azide resistance
• NalR = Nalidix.acidResist.
• strR = streptomycin resist.

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