CGSC Strain#: 5487
Strain Designation: K146 Thy-      Source of Strain: F. Stoeber
Sex: F- Chromosomal Markers: fhuA2::IS2, lacY1, lacZ4, lac-20, tsx-7, glnX44(AS), gal-6, hisG1, thyA58, rpsL17, rpsL8, malT1(λ^R), kdgK1, metB1, ΔargH1, rplL9(L?), thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• thyA58-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• = strep-resistant
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant

References: