CGSC Strain#: 6400
Strain Designation: UTH4314      Source of Strain: E.P. Goldschmidt
Other Designations: AC6400
Sex: F- Chromosomal Markers: hisD4314, thyA321, deo-71
Strain Comments:

• thyA321-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.

References:

• Goldschmidt, E.P., M.S. Cater, T.S. Matney, M.A. Butler, A. Greene 1970. Genetic analysis of the histidine operon in Escherichia coli K12. Genetics 66:219-229