CGSC Strain#: 7707
Strain Designation: AW405      
Source of Strain: J. Adler
Sex: F- 
 Chromosomal Markers: thr-1, 
araC14, 
leuB6(Am)
, 
fhuA31, 
lacY1, 
tsx-78, 
glnX44(AS)
, 
galK2(Oc)
, 
galT22, 
λ-, 
hisG4(Oc)
, 
rfbC1, 
mgl-51, 
rpsL136(strR)
, 
xylA5, 
mtl-1, 
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)                                                                                                                                                                                                                          
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.                                                                                                          
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References:- Hazelbauer, GL, RE Mesibov, J Adler 1969. Escherichia coli mutants defective in chemotaxis toward specific chemicals. Proc. Natl. Acad. Sci. U.S.A. 64(4):1300-7.
- Parkinson, J.S. 1980. Novel mutations affecting a signaling component for chemotaxis of Escherichia coli. J.Bacteriol. 142:953-961