CGSC Strain#: 14238
Strain Designation: WTZ2204      Source of Strain: J.E. Wertz
Sex: F- Chromosomal Markers: λ^-, ΔfliC769::kan, rph-1
Strain Comments:

• Made by P1 transduction of the fliC knockout from JW1908-1 into MG1655 (CGSC 6300)
• ΔfliC769::kan-- The primers used to make this knockout were GTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGATTCCGGGGATCCGTCGACC and GGAAACCCAATACGTAATCAACGACTTGCAATATAGGATAACGAATCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References: