CGSC Strain#: 14234
Strain Designation: WTZ19002      Source of Strain: J.E. Wertz
Other Designations: E. coli 15 T- /pUC18
Sex: F- Episome/Plasmid: pUC18
   Plasmid Markers/Mutations: ori_pMB1, lacZα, bla
   Plasmid Comment: Cloning vector
Chromosomal Markers: thyA42, deoB20
Strain Comments:

• Made by transforming pUC18 into CGSC 4906
• This is mutant #376 of Roepke, received from S.S. Cohen.
• thyA42-- E. coli 15 mutation
• thyA42-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• deoB20-- E. coli 15 mutation.
• deoB20 was formerly called thyR32

References:

• Roepke, R R, R L Libby, M H Small 1944. Mutation or Variation of Escherichia coli with Respect to Growth Requirements. 48(4):401-12.
• MUKAI, FH 1960. Interrelationship between colicin sensitivity and phage resistance. J. Gen. Microbiol. 23:539-51.