CGSC Strain#: 14233
Strain Designation: WTZ19001     
Source of Strain: J.E. Wertz
Other Designations: JK378 - rsgA
Sex: F-
Chromosomal Markers: ara-600,
Δ(gpt-lac)5,
λ-,
zdi-925::Tn10,
infC362,
relA1,
spoT1,
thiE1,
ΔrsgA787::kanStrain Comments: - Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- zdi-925::Tn10-- cotransduces ca. 65% with pps and 55% with pfkB. Found by PCR (Nichols et al. 1998) to be an insertion at nucleotide 1,776,386 (38.291 min.)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- ΔrsgA787::kan-- The primers used to make this knockout were GACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAATTCCGGGGATCCGTCGACC and GTATCATAGATGTTTTGCCCATCAGGGGCGACCAGGAGTCTGTACGATTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- thiE1 was formerly called thi-1
- = tetracycline resistant
- = kanamycin resistant
References: - Sussman, J.K., E.L. Simons, R.W. Simons 1996. Escherichia coli translation initiation factor 3 discriminates the initiation codon in vivo. Mol.Microbiol. 21:347-360