CGSC Strain#: 14228
Strain Designation: WTZ18004      Source of Strain: J.E. Wertz
Sex: F- Chromosomal Markers: Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔrelA882::FRT, rph-1, ΔbipA733::kan, Δ(rhaD-rhaB)568, hsdR514
Strain Comments:

• This is a double knockout constructed from Keio strains by the CGSC
• This strain should be grown at 37 deg. C and tends to grow slowly. Growth at lower temperatures will tend to select for rluC mutations that suppress the cold sensitive phenotype of the bipA deletion.
• Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
• ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
• ΔrelA882::FRT-- This is the ?relA782::kan allele with the kan cassette removed leaving an FRT scar
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• ΔbipA733::kan-- The primers used to make this knockout were GGCAGGTTTTTTATGACTAAAAAACGAAATTAATCGTCTTTCGGTGCGCGATTCCGGGGATCCGTCGACC and TAATCCGTGTACAATAACGCGCTATTTCTAATGCCTGAGGCAAAGTTGTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• Δ(araD-araB)567 was formerly called Δ araBAD_AH33
• Δ(rhaD-rhaB)568 was formerly called Δ rhaBAD_LD78
• ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
• ΔlacZ4787(::rrnB-3) was formerly called Δ lacZ_WJ16
• = kanamycin resistant
• ::rrnB-3 = 4 copies of rrnB inserted

References:

• Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11