CGSC Strain#: 13655
Strain Designation: pLC32-38
Source of Strain: Sex: F+
Episome/Plasmid: pLC32-38
Plasmid Markers/Mutations: araC14,
thr-1,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1,
argR ,
mdh Plasmid Comment: Hybridizes with Kohara clones 525, 526, and 527. Carries argR and mdh at 72.9 minutes.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA56-- Amino acid substitution Arg 60 Cys
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Eckhardt, T. 1980. Isolation of plasmids carrying the arginine repressor gene argR of Escherichia coli K12. Mol.Gen.Genet. 178:447-452
- Sutherland, P., L. McAlister-Henn 1985. Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase. J.Bacteriol. 163:1074-1077
- Vogel, R.F., K.-D. Entian, D. Mecke 1987. Cloning and sequence of the mdh structural gene of Escherichia coli coding for malate dehydrogenase. Arch.Microbiol. 149:36-42
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151