CGSC Strain#: 14210
Strain Designation: WTZ17007      Source of Strain: J.E. Wertz
Sex: F- Chromosomal Markers: ΔaraC771::kan, λ^-, rph-1
Strain Comments:

• ΔaraC771::kan-- The primers used to make this knockout were AATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGATTCCGGGGATCCGTCGACC and AAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

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