CGSC Strain#: 6150
Strain Designation: GS20      Source of Strain: T.J. Silhavy
Sex: F- Chromosomal Markers: Δ(gpt-lac)5, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rpoB324(rifR), thiE1, metA28, lamB20::Tn5, deoC1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• thiE1 was formerly called thi-1
• = kanamycin resistant
• fruA25 = former designation
• rifR = rifamycin resistance
• strR = streptomycin resist.

References:

• Guarente, L.P., R.R. Isberg, M. Syvanen, T.J. Silhavy 1980. Conferral of transposable properties to a chromosomal gene in Escherichia coli. J.Mol.Biol. 141:235-248