CGSC Strain#: 5897
Strain Designation: E835      Source of Strain: B. Egan
Other Designations: RW870
Sex: F- Chromosomal Markers: galK2(Oc), λ^-, recA3, recB21, rpsL200(strR)
Strain Comments:

• The recA allele is incorrectly given as recA56 in the paper cited.
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Pilarksi, LM, JB Egan 1973. Role of DNA topology in transcription of coliphage lambda in vivo. II. DNA topology protects the template from exonuclease attack. J. Mol. Biol. 76(2):257-66.