CGSC Strain#: 5700
Strain Designation: EC-0,strA24,rif123      Source of Strain: Chakrabarti & Gorini
Sex: F' Episome/Plasmid: F128
   Plasmid Markers/Mutations:
Chromosomal Markers: Δ(gpt-lac)5, glnX44(AS), λ^-, relA1, rpsL214(strR), rpoB23(rifR), thiE1
Strain Comments:

• This strain should be maintained on a medium containing thiamin and with lactose as the carbon source.
• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• rifR = rifamycin resistance
• strR = streptomycin resist.

References:

• Chakrabarti, SL, L Gorini 1975. A link between streptomycin and rifampicin mutation. Proc. Natl. Acad. Sci. U.S.A. 72(6):2084-7.
• Chakrabarti, SL, L Gorini 1977. Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination. Proc. Natl. Acad. Sci. U.S.A. 74(3):1157-61.