CGSC Strain#: 5622
Strain Designation: WA3057      Source of Strain: R. Curtiss
Sex: F- Chromosomal Markers: trpE68, thyA42, metE101, deoB20, arg-69, hsdA+
Strain Comments:

• Could not find description cards; sent pedigree.
• thyA42-- E. coli 15 mutation
• thyA42-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• deoB20-- E. coli 15 mutation.
• deoB20 was formerly called thyR32

References: