CGSC Strain#: 14198
Strain Designation: WTZ16003      Source of Strain: J.E. Wertz
Sex: F- Chromosomal Markers: ΔfhuA866::FRT, ΔphoA848::FRT, λ^-, IN(rrnD-rrnE)1, rph-1
Strain Comments:

• W3110 with phoA and fhuA deletions transduced from the Keio collection and then the kan cassettes removed.
• ΔphoA848::FRT-- This is the ΔphoA748::kan allele with the kan cassette removed leaving an FRT scar
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

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