CGSC Strain#: 5246
Strain Designation: G13      Source of Strain: A. Garen
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, phoR51, relA1, pitA10, spoT1, phoT-35, T2R
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• phoT-35-- is a phoS or phoT mutation.
• T2R-- : selected for resistance to phage T2

References:

• Echols, H., A. Garen, A. Torriani 1961. Genetic control of repression of alkaline phosphatase in E. coli. J.Mol.Biol. 3:425-438
• Kreuzer, K.N., C. Pratt, A. Torriani 1975. Genetic analysis of regulatory mutants of alkaline phosphatase of Escherichia coli. Genetics 81:459-468