CGSC Strain#: 14193
Strain Designation: WTZ14193      Source of Strain: J.E. Wertz
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), pflB1, ldhA9, rfbC1, ΔrecA635::kan, rpsL175(strR), thiE1
Strain Comments:

• ldh pfl double mutants do not grow anaerobically on any sugar or sugar alcohol even in presence of acetate.
• This is a recA- version of FMJ39
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• ldhA9 was formerly called ldh-1
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = kanamycin resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.

References:

• Mat-Jan, F., K.Y. Alam, D.P. Clark 1989. Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase. J.Bacteriol. 171:342-348