CGSC Strain#: 5027
Strain Designation: JC5412      Source of Strain:
Sex: F- Chromosomal Markers: thr-300, λ^-, sbcA8(Rac-8 del.), relA1, recB21, rpsE2300(SpcR), spoT1, ilv-318, thiE1
Strain Comments:

• laboratory, where it was checked thoroughly. You may wish to substitute it for the sample sent to you previously.
• The culture of JC5412 which has been sent out as CGSC #5027 was Trp^-, which it should not have been. The sample of JC5412 enclosed came from a culture which was just received from A.J. Clark's
• sbcA8(Rac-8 del.)-- is a deletion that removes a large part of rac and extends into the bacterial chromosome.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• sbcA8(Rac-8 del.) was formerly called sbcA8
• thiE1 was formerly called thi-1
• Rac-8 del. = may delete ca. 1/2 of Rac
• SpcR = spectinomycin resistant

References:

• Clark, A.J. 1967. The beginning of a genetic analysis of recombination proficiency. J.Cell.Physiol. 70:165-180
• Willetts, N.S., D.W. Mount 1969. Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations contrasducible with thyA. J.Bacteriol. 100:923-934
• Willetts, N.S., A.J. Clark 1969. Characteristics of some multiply recombination-deficient strains of Escherichia coli. J.Bacteriol. 100:231-239