CGSC Strain#: 14186
Strain Designation: WTZ15002      Source of Strain: J.E. Wertz
Sex: F+ Chromosomal Markers: asnB32, λ^-, ΔgdhA859::FRT, relA1, ΔgltB740::kan, spoT1, asnA31, thiE1
Strain Comments:

• This strain requires 100ug/ml L-asparagine and 100ug/ml L-glutamate
• ΔgdhA859::FRT-- This is the ΔgdhA759::kan allele with the kan cassette removed leaving an FRT scar
• ΔgltB740::kan-- The primers used to make this knockout were TAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGATTCCGGGGATCCGTCGACC and TAAACATTCTGACTCATTGTTGCTACCCCTTACTGCGCCTGCACGCGCAATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• asnA31-- Reported to be an amber mutation
• thiE1 was formerly called thi-1
• = kanamycin resistant
• del-ins = deletion-insertion

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