CGSC Strain#: 4403
Strain Designation: 2320      Source of Strain: J. Monod
Other Designations: EMG12
Sex: F- Chromosomal Markers: lacZ13(Oc), lacI22, glnX44(AS), λ^-, rpsL135(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• This strain is phenotypically z^- and y^-.
• lacZ13(Oc)-- is lacZ₂ of Jacob, originating in Paris strain 3.320. It is an ochre, polar mutation.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• lacI22 was formerly called lac_i3^-
• lacI22 was formerly called lacI3
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Franklin, N.C., S.E. Luria 1961. Transduction by bacteriophage P1 and the properties of the lac genetic region in E. coli and S. dysenteriae. Virology 15:299-311
• Brenner, S., J.R. Beckwith 1965. Ochre mutants, a new class of suppressible nonsense mutants. J.Mol.Biol. 13:629-637
• Clowes, R., W. Hayes 1968. Experiments in microbial genetics