CGSC Strain#: 4364
Strain Designation: KL217      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: galK35, λ^-, pyrD34, trpC45, his-68, gyrA12(NalR), recA1, argG34, metB1, thr-21
Strain Comments:

• This strain may contain more than one arg^- mutation. Due to misinformation sent out by the CGSC, some of the allele designations are given incorrectly in the reference.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• NalR = Nalidix.acidResist.

References:

• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812