CGSC Strain#: 14174
Strain Designation: MG1655/pWTZ594      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: pWTZ594
   Plasmid Markers/Mutations: bla(ApR), lacZp-GFPuv(gfp), ori[pMB1]
   Plasmid Comment: This plasmid was constructed by fusing the GFPuv gene behind the lacZ promoter in pBluescript KS+
Chromosomal Markers: λ^-, rph-1
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• ApR = ampicillin resistant
• gfp = green fluorescent protein

References:

• Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.