CGSC Strain#: 14176
Strain Designation: BW25993/pKD46      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: pKD46
   Plasmid Markers/Mutations: repA101(ts), araBp-gam-bet-exo, oriR101, bla(ApR), araC , bla , [tL3]
   Plasmid Comment: Lambda Red recombinase expression plasmid -- includes 2154 nucleotides (31088-33241) of phage Lambda, which includes the terminator downstream frp, exo, tL3 and a small ORF.GenBk-AY048746
Chromosomal Markers: Δ(araD-araB)567, λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514
Strain Comments:

• Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(araD-araB)567 was formerly called Δ araBAD_AH33
• Δ(rhaD-rhaB)568 was formerly called Δ rhaBAD_LD78
• ApR = ampicillin resistant
• ts = Temperature Sensitive

References:

• Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.